6/19/2023 0 Comments Vmd networkview![]() U bound to TDG82-308 (1.54 A) and TDG ' 111-308 (1.71 A), revealing ' new enzyme-substrate contacts, direct and watermediated.We report the first high-resolution structures of TDG in an enzyme-substrate complex, for G T substrate affinity and glycosylase activity of TDG82-308 greatly exceeds that of TDG111-308 and is equivalent to full-length TDG.Crystal structures and NMR experiments demonstrate that most of these N-terminal residues are disordered, more ยป for substrate- or productbound TDG82-308. ![]() ![]() Here, we report structural and functional studies of TDG82-308, a new construct containing 29 more N-terminal residues than TDG111-308, the construct used for previous structures of DNAbound TDG. Essential for DNA demethylation, TDG excises 5- formylcytosine and 5-carboxylcytosine, derivatives of mC generated by Tet (ten-eleven translocation) enzymes. T mispairs arising from deamination of 5-methylcytosine (mC), and it processes other deamination-derived lesions including uracil (U).Thymine DNA Glycosylase (TDG) is a base excision repair enzyme functioning in DNA repair and epigenetic regulation. National Energy Research Scientific Computing Center (NERSC) Sponsoring Org.: USDOE Office of Science (SC) National Institutes of Health (NIH) National Science Foundation (NSF) OSTI Identifier: 1577474 Alternate Identifier(s): OSTI ID: 1636021 Grant/Contract Number: AC02-05CH11231 GM013306 GM110387 CHE110042 Resource Type: Journal Article: Accepted Manuscript Journal Name: Journal of Molecular Biology Additional Journal Information: Journal Volume: 431 Journal Issue: 14 Journal ID: ISSN 0022-2836 Publisher: Elsevier Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES biochemistry & molecular biology proliferating cell nuclear antigen replication factor C DNA damage response molecular dynamics binding energy Publication Date: Research Org.: Lawrence Berkeley National Lab. Georgia State Univ., Atlanta, GA (United States).Pennsylvania State Univ., University Park, PA (United States).
0 Comments
Leave a Reply. |